Diversity in CD8+ T Cell Function and Epitope Breadth Among Persons with Genital Herpes
Identifieur interne : 002726 ( Main/Exploration ); précédent : 002725; suivant : 002727Diversity in CD8+ T Cell Function and Epitope Breadth Among Persons with Genital Herpes
Auteurs : Kerry J. Laing [États-Unis] ; Amalia S. Magaret [États-Unis] ; Dawn E. Mueller [États-Unis] ; Lin Zhao [États-Unis] ; Christine Johnston [États-Unis] ; Stephen C. De Rosa [États-Unis] ; David M. Koelle [États-Unis] ; Anna Wald [États-Unis] ; Lawrence Corey [États-Unis]Source :
- Journal of Clinical Immunology [ 0271-9142 ] ; 2010-09-01.
English descriptors
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Abstract
Abstract: CD8+ T cells are known to be important in clearing herpes simplex virus (HSV) infections. However, investigating the specific antiviral mechanisms employed by HSV-2-specific T cell populations is limited by a lack of reagents such as CD8+ T cell epitopes and specific tetramers. Using a combination of intracellular cytokine staining flow cytometry and ELISpot methods, we functionally characterized peripheral HSV-2-specific CD8+ T cells from peripheral blood mononuclear cell (PBMC) that recognize 14 selected HSV-2 open-reading frames (ORFs) from 55 HSV-2 seropositive persons; within these ORFs, we subsequently identified more than 20 unique CD8+ T cell epitopes. CD8+ T cells to HSV-2 exhibited significant heterogeneity in their functional characteristics, proliferation, production of inflammatory cytokines, and potential to degranulate ex vivo. The diversity in T cell response in these ex vivo assessments offers the potential of defining immune correlates of HSV-2 reactivation in humans.
Url:
DOI: 10.1007/s10875-010-9441-2
Affiliations:
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Box 19024, LE-500, 98109-1024, Seattle, WA</wicri:regionArea><wicri:noRegion>WA</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Clinical Immunology</title><title level="j" type="abbrev">J Clin Immunol</title><idno type="ISSN">0271-9142</idno><idno type="eISSN">1573-2592</idno><imprint><publisher>Springer US; http://www.springer-ny.com</publisher><pubPlace>Boston</pubPlace><date type="published" when="2010-09-01">2010-09-01</date><biblScope unit="volume">30</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="703">703</biblScope><biblScope unit="page" to="722">722</biblScope></imprint><idno type="ISSN">0271-9142</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0271-9142</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>HSV-2</term><term>T cells</term><term>antigens/peptides/epitopes</term><term>virus</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: CD8+ T cells are known to be important in clearing herpes simplex virus (HSV) infections. However, investigating the specific antiviral mechanisms employed by HSV-2-specific T cell populations is limited by a lack of reagents such as CD8+ T cell epitopes and specific tetramers. Using a combination of intracellular cytokine staining flow cytometry and ELISpot methods, we functionally characterized peripheral HSV-2-specific CD8+ T cells from peripheral blood mononuclear cell (PBMC) that recognize 14 selected HSV-2 open-reading frames (ORFs) from 55 HSV-2 seropositive persons; within these ORFs, we subsequently identified more than 20 unique CD8+ T cell epitopes. CD8+ T cells to HSV-2 exhibited significant heterogeneity in their functional characteristics, proliferation, production of inflammatory cytokines, and potential to degranulate ex vivo. The diversity in T cell response in these ex vivo assessments offers the potential of defining immune correlates of HSV-2 reactivation in humans.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Washington (État)</li></region><settlement><li>Seattle</li></settlement><orgName><li>Université de Washington</li></orgName></list><tree><country name="États-Unis"><noRegion><name sortKey="Laing, Kerry J" sort="Laing, Kerry J" uniqKey="Laing K" first="Kerry J." last="Laing">Kerry J. Laing</name></noRegion><name sortKey="Corey, Lawrence" sort="Corey, Lawrence" uniqKey="Corey L" first="Lawrence" last="Corey">Lawrence Corey</name><name sortKey="Corey, Lawrence" sort="Corey, Lawrence" uniqKey="Corey L" first="Lawrence" last="Corey">Lawrence Corey</name><name sortKey="Corey, Lawrence" sort="Corey, Lawrence" uniqKey="Corey L" first="Lawrence" last="Corey">Lawrence Corey</name><name sortKey="Corey, Lawrence" sort="Corey, Lawrence" uniqKey="Corey L" first="Lawrence" last="Corey">Lawrence Corey</name><name sortKey="Corey, Lawrence" sort="Corey, Lawrence" uniqKey="Corey L" first="Lawrence" last="Corey">Lawrence Corey</name><name sortKey="De Rosa, Stephen C" sort="De Rosa, Stephen C" uniqKey="De Rosa S" first="Stephen C." last="De Rosa">Stephen C. De Rosa</name><name sortKey="De Rosa, Stephen C" sort="De Rosa, Stephen C" uniqKey="De Rosa S" first="Stephen C." last="De Rosa">Stephen C. De Rosa</name><name sortKey="De Rosa, Stephen C" sort="De Rosa, Stephen C" uniqKey="De Rosa S" first="Stephen C." last="De Rosa">Stephen C. De Rosa</name><name sortKey="De Rosa, Stephen C" sort="De Rosa, Stephen C" uniqKey="De Rosa S" first="Stephen C." last="De Rosa">Stephen C. De Rosa</name><name sortKey="Johnston, Christine" sort="Johnston, Christine" uniqKey="Johnston C" first="Christine" last="Johnston">Christine Johnston</name><name sortKey="Koelle, David M" sort="Koelle, David M" uniqKey="Koelle D" first="David M." last="Koelle">David M. Koelle</name><name sortKey="Koelle, David M" sort="Koelle, David M" uniqKey="Koelle D" first="David M." last="Koelle">David M. Koelle</name><name sortKey="Koelle, David M" sort="Koelle, David M" uniqKey="Koelle D" first="David M." last="Koelle">David M. Koelle</name><name sortKey="Koelle, David M" sort="Koelle, David M" uniqKey="Koelle D" first="David M." last="Koelle">David M. Koelle</name><name sortKey="Koelle, David M" sort="Koelle, David M" uniqKey="Koelle D" first="David M." last="Koelle">David M. Koelle</name><name sortKey="Magaret, Amalia S" sort="Magaret, Amalia S" uniqKey="Magaret A" first="Amalia S." last="Magaret">Amalia S. Magaret</name><name sortKey="Magaret, Amalia S" sort="Magaret, Amalia S" uniqKey="Magaret A" first="Amalia S." last="Magaret">Amalia S. Magaret</name><name sortKey="Mueller, Dawn E" sort="Mueller, Dawn E" uniqKey="Mueller D" first="Dawn E." last="Mueller">Dawn E. Mueller</name><name sortKey="Wald, Anna" sort="Wald, Anna" uniqKey="Wald A" first="Anna" last="Wald">Anna Wald</name><name sortKey="Wald, Anna" sort="Wald, Anna" uniqKey="Wald A" first="Anna" last="Wald">Anna Wald</name><name sortKey="Wald, Anna" sort="Wald, Anna" uniqKey="Wald A" first="Anna" last="Wald">Anna Wald</name><name sortKey="Wald, Anna" sort="Wald, Anna" uniqKey="Wald A" first="Anna" last="Wald">Anna Wald</name><name sortKey="Zhao, Lin" sort="Zhao, Lin" uniqKey="Zhao L" first="Lin" last="Zhao">Lin Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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